National Taiwan University College of Medicine

According to different academic areas including:

Spectroscopy applied to tissue diagnosis

The pathological diagnosis of the disease is mainly analyzed by biopsy in living organs, and then by the microscope analysis for morphological variability of tissue structures and cells. Due to optical biopsy contains the advantages of real-time disease diagnosis, straightforward tissue obtaining and direct physiological parameters measurement, in recent years, optical diagnosis technology thus become an important part of the optoelectronic technology development. The center currently focus on the development of photo-excited auto-fluorescence spectroscopy or dye-additional fluorescence spectra as supportive diagnosis tools of early tissue pathological changes and as biomarker studies after treatment. It is expected that the fluorescence spectra changes by auto-fluorescence substances or additional photosensitizers can locate the tissue pathologies or cancer lesions.

Photodynamic therapy on various treatments of cancer

The basic principle of photodynamic therapy is excited by specific wavelengths of laser light with external photosensitizers on tissue, and light energy can be transferred to tissues by occurring photochemical effect with substances such as oxygen. After that, it turns to produce toxic free radicals on cell, so the location accumulated of photosensitizers can cause cell toxicity after the light exposure. The photodynamic therapy constitutes three basic elements, which are photosensitizer, light and oxygen in the cells. Because of the need to produce treatment effect under these three elements corporation, laser photodynamic therapy is different from the traditional laser surgery such as simple excision, cauterization or vaporization.

The mechanism research of photodynamic lethal effect and resistance molecular

Combination of optoelectronic technology and related works in cell biology can aid to research the mechanism of molecular biological effects caused by photodynamic effects. The key point of this study is to explore how the triggered photodynamic effects lead to cell apoptosis when the photosensitizers are excited by light. At present, researches focus on the signal transduction caused by photodynamic effects. And those triggered messengers whether would prevent cell division or promote apoptosis by controlling mitotic factors. Through the exploration of these mechanisms, we can propose further treatments for photodynamic-resistant cells may produce in the future.

Study on brain function by magnetic resonance imaging (MRI)

Development of water molecule diffusion tensor magnetic resonance imaging (DTI) technology: this technology can illustrate the fiber strike of cerebral white matter in 3D space with non-invasive instruments. And then it utilize 3D tracking mathematical model to reorganize the connection of axons between gray matter and nerve nucleus. This technique can be used to study the structure of neural connection in 3D space. In addition, it can analyze the extent of damage to nerve fiber through white matter lesions and compare with the clinical symptoms.

Development of visual and motor functional magnetic resonance imaging (FMRI): FMRI provides particular response image when each functional area of brain gray matter is stimulated. If this technique combines with the water molecule DTI, we can study the features of functional areas connected with each other. We will overcome some difficulties of combining these two techniques through studying the connection condition between the brain's primary visual areas and minor visual areas connected with each other. This research can help us understand how the visual areas of the brain gray matter do the functional integration through the white matter fibers.

Study on heart function by magnetic resonance imaging (MRI)

Development of water molecule DTI on cardiac muscle fiber visualization in vivo: we use this technique to explore the cardiac hypertrophy caused by various reasons, the different arrangements on cardiac muscle fibers between them, as well as the effects on ventricular function between these various changes. In addition, we also study how to remodel cardiac muscle fiber arrangement under drug therapy.

Development of phase-contrast techniques to measure cardiac strain tensor: combination this technique with water molecule DTI can study the relationship between cardiac systolic/diastolic function and myocardial structure. This research provides ventricular mechanics the most basic and important data. We can obtain myocardial strain tensor and myocardial fiber orientation data by these two techniques, and referring substances visco-elastic coefficient to calculate myocardial stress distribution and ventricular cavity pressures. After that, we can compare them with the measurement results of cardiac catheter pressure gauge.

Molecular mechanism analysis on myocardium or vessel walls shape reconstruction in compressive condition

We took advantage of MRI regularly tracking stress and strain tensors of myocardium or arterial vessel wall of spontaneously hypertensive rats and observe the changes on internal tissue structure and external morphology. To further understand the internal changes under pressure effect, we intend to combine the study on medical engineering with molecular biology in advance to explore the intracellular changes of signal transduction and the mechanism how cell growth and death triggered under the pressure.

Development of antibody microarrays and optical sensing technology

In the future, the study of antibody microarray will focus on research and development of "Opto-BioMorphin—Multi-Functional Optical Biochip System" in the next generation. And "antibody microarray" will be the subject of the research. Among the early phase we can adopt the surrounding support system as a vehicle which consolidates the common features of different chips to develop a real-time and multifunction photoelectric detection system. And it contains photoelectric detection feature of ellipsometer. Once completed, it will be applied to not only biomedical chip for biochemical detection, but also examination of chip mass production. In addition, we will also construct Opto-BioMorphin related bio-computer interface technology, and develop organic synthesis of different carbon, different endpoint functional group interface connection substances to simulation "immobilized proteins" as a highly mobile "free protein in solution". And achieve the standard protein chip with features of location, orientation, high binding capacity and low costs.

Research and development of macro biomolecules structure and interatomic bonds microscopy

Biomolecules structure and interatomic bonds microscopy technology development mainly focus on watching structural changes associated with biological activity and macro biomolecules 3D map. So the sample preparation procedure development, maintenance of biological functions and interatomic bonds molecular imaging are critical key points of research and development. The key technologies of study mainly focus on bio-molecular interaction between samples with a fixed support. Any molecular structure collapse caused by surface tension during the dry period, heat collapse caused by freeze-drying, and shape distortion caused by embedding giant molecular are biological factors for research in the future.

Piezoelectric Bio-sensing technology research

Piezoelectric Bio-sensing technology development will focus on using quartz crystal microbalance (QCM) to explore antibody-antigen interaction dynamic theoretical model and validate with experiment in the future. Monoclonal and polyclonal antibody and their compensate antigen are used as a model system without any molecular modification or linkage. The mass changes between two molecules and corresponding mode of piezoelectric vibration frequency change will be observed. Dynamics between the various constituent molecules interrelatedness will be established. And their real affinity constant will be estimated, during which the interaction of affinity will be proved. The value would be confirmed by traditional methods to speed up the establishment of cornerstone on commercialization of patented of QCM piezoelectric biosensor for the coming years.